Probiotics and cellular interactions
✔ Optimise the development and quality of your probiotic-based products with our range of microbiology analytical services !
👉Development, validation, transfer, suitability and microbiological control of raw materials, bulks, finished products and packaging items.
ACM Pharma Fonderephar adapts to each need
The laboratory is equiped with specific equipment and real expertise in the detection and identification of microaerophilic and strict anaerobic flora, cell culture, and immune responses.
These tests are carried out as part of partnerships with Clinical Research Centers or hospital or private Clinical Investigation Centers.
FONDEREPHAR is not involved in the recruitment of subjects, nor in the sampling stage.
- Microbiological analyses:
- Study of human (or animal) skin, oral, pharyngeal, intestinal, and vaginal floras:
- Quantitative evaluation by culture of the flora of interest according to internal or client method: Lactobacillus spe, Bifidobacterium sp, Saccharomyces cerevisiae var boulardii…
- Qualitative evaluation by MALDI TOF microbial isolation and identification or by Molecular Biology.
- Study of human (or animal) skin, oral, pharyngeal, intestinal, and vaginal floras:
- Evaluation of the growth delay of pathogenic strains in the presence of probiotics (in liquid or gelled medium)
- Demonstration of prebiotic effect
FONDEREPHAR has a cell culture platform for the performance of in vitro tests:
- Evaluation of the cellular cytotoxicity of cosmetic products (ISO 10993-5, MTT method, phenol red)
- Ex vivo tests on skin explants
- Evaluation of the interaction of microorganisms/Eukaryotic cells
- Cellular adhesion of probiotics
- Cellular adhesion of “undesirable” strains
- Inhibition of the adhesion of “undesirable” strains to these same cells in the presence of cosmetic product
- Detaching effect of “undesirable” strains from cosmetic products
Examples of applications:
- Evaluation of antimicrobial activity
- Evaluation of probiotic/prebiotic activity
- Control of flora balance
Example of strains and test cells :
- VAESBJk keratinocyte: Staphylococcus aureus
- Gastric epithelial cell (NCI-N87): Cutibacterium acne
- Nasal cell CCL-30: Corynebacterium xerosis
- HUMAN SKIN XPLANTS: Helicobacter pylori
- Caco2 colorectal cell: Escherichia coli
- HMVII vaginal cell: Malassezia furfur, Branhamella catharralis, Candida albicans, Streptococcus pyogenes
Our expertise
Carrying out in vitro studies on a wide range of cell lines, isolated cells or primo-cultures from humans or animals in the Pharmaceuticals and Cosmetics fields
The laboratory has specific equipment and human resources specialising in the detection and identification of microaerophilic and strict anaerobic flora.
At your service, we identify your needs and implement our expertise for an adapted, efficient and tailor-made service.